IDENTIFILER DIRECT PDF
AmpFlSTR® Identifiler® Direct PCR. Amplification Kit for use with: reaction kit (Part no. ). reaction kit (Part no. ). Publication Part. Development and validation of the AmpFℓSTR® Identifiler® Direct PCR Amplification Kit: a multiplex assay for the direct amplification of. The AmpFℓSTR® Identifiler® Direct PCR Amplification Kit has been developed specifically to help address the DNA extraction and purification bottleneck that.
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Multicomponent analysis is the process that separates the five different fluorescent dye colors into distinct spectral components.
AmpFlSTR Identifiler Direct PCR Amplification Kit – PDF
C Revision Date May Contents. The fluorescent dyes attached to the primers are light-sensitive. This sample type is not frequently used for the collection of database or casework reference samples. The data collection software stores information about each sample in a sample file.
General Considerations 3 3.
Seed cells in plate format. Before using this product, read and understand the information the Safety appendix in this document. This experiment accounts for instrument-to-instrument and sample-to-sample variations. Careful optimization of the cycle number will help to minimize this variation.
Information in this document is subject to change without notice. Place the tip of a 1.
Data collection identifkler analysis software Instrument and software compatibility The data collection software provides instructions to firmware running on the instrument and displays instrument status and raw data in real time.
See Allelic ladder requirements on page 28 for information on ensuring accurate genotyping. About multicomponent analysis Life Technologies fluorescent multi-color dye technology allows the analysis of multiple loci, including loci that have alleles with overlapping size ranges.
Sample Protocol for Adherent Cells. To learn practical applications of DNA purification. Prepare sufficient PCR reagents to complete amplification of three replicate plates. Materials to be Supplied More information.
All technical literature is available on the Internet at Iventifiler information. Due idehtifiler overlap More information. Version 1 Last Updated 1. Data is therefore collected More information. User manual Version More information.
New Features in v5. For safety and biohazard guidelines. Promega Corporation, Updated September Radioactive label, silver More information. During data collection on Life Technologies instruments, the fluorescence signals are separated by a diffraction grating according to their wavelengths and projected onto a charge-coupled device CCD camera in a predictably spaced pattern.
To maximize result quality, prepare and amplify Plate 1 then repeat for Plates 2 and 3. Ensure the selected samples represent a identitiler range of samples analyzed in your laboratory.
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See Table 1 on page 10 for direch list of alleles included in the allelic ladder. Periodically, optimizations and revisions are made to the kit. Use of this thermal cycling platform may adversely affect performance of the Identifiler Direct Kit.
Not for identifuler in diagnostic More information. GoClone Repor ter Construc ts: Technical Focus Being Direct: Classic Immunoprecipitation PR 01 G-Biosciences technical gbiosciences. If using the thermal cycler with silver or gold-plated silver block and adhesive clear film instead of caps to seal the plate wells, place a MicroAmp compression pad Part no on top of the plate to prevent evaporation during thermal cycling.
G Institute of Applied Biotechnologies a. Create three identical PCR plates see page for a suggested plate layout.
Table 1 shows the loci amplified, their chromosomal locations, and the corresponding fluorescent marker dyes. To minimize the effect of instrument-to-instrument variation, use the same thermal cycler to amplify all three plates.
AmpFlSTR Identifiler Direct PCR Amplification Kit
The goal of multicomponent analysis is to correct for spectral overlap. Increasing identiffiler size of the punch may cause inhibition during PCR amplification. Administrator s Guide More information.
Windows and Windows Vista are trademarks of Microsoft Corporation. Determine optimum conditions 1.